Anti-Myosin Light Chain 2 antibody ab79935 from Abcam

Product Specs
ItemAnti-Myosin Light Chain 2 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Supplier Product Page
Catalog Numberab79935
Antibody TypePrimary
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
HostRabbit
Conjugate/TagUnconjugated
IsotypeIgG
Quantity100 UG
ClonalityPolyclonal
Concentration0.7 mg/ml
Gene NameMYL2
ReactivityHuman, Mouse, Rat
TargetMyosin Light Chain 2

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Website: http://www.abcam.com

(1) Biophysical properties of NaV1.5 channels from atrial-like and ventricular-like cardiomyocytes derived from human induced pluripotent stem cellsScientific ReportsNovember 24, 2023Charles-Albert Chapotte-Baldacci, Marion Pierre, Mohammed Djemai, Valérie Pouliot, Mohamed Chahine(1:400, Cat# ab68086, Abcam, RRID:AB_1140497), rabbit anti-myosin light chain 2 (MYL2) (1:2000, Cat# ab79935, Abcam, RRID:AB_1952220), mouse anti-TNNT2 (1:5000, Cat# ab10214, Abcam, RRID:AB_2206574), rabbit anti-gap

(2) Utilizing human induced pluripotent stem cells to study atrial arrhythmias in the short QT syndromeJournal of Molecular and Cellular CardiologyOctober 1, 2023Assad Shiti, Gil Arbil, Naim Shaheen, Irit Huber, Noga Setter, Lior Gepsteinthe unconjugated primary antibodies targeting cTnT (MA5–12960, Thermo-Fisher Scientific) and MLC-2 V (ab79935, Abcam) at 1:500 dilution in flow-cytometry buffer consisting of PBS with 0.5% fetal bovine serum (FBS,

(3) Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigsNPJ Regenerative medicineMay 26, 2023Yap L, Chong LY, Tan C, Adusumalli S, Seow M, Guo J, Cai Z, Loo SJ, Lim E, Tan RS, et al.2180 S, RRID:AB_2218736, 1:300), anti-Ku80 conjugated 488 (Abcam, ab198586, 1:100), anti-MLC2v (Abcam, ab79935, RRID:AB_1952220, 1:100), anti-ACTN2 (Sigma, A2172, RRID:AB_476695 1:500), anti-N-cadherin (Sigma, C3678,

(4) Molecular characterization of the intact mouse muscle spindle using a multi-omics approacheLifeFebruary 6, 2023Bavat Bornstein, Lia Heinemann-Yerushalmi, Sharon Krief, Ruth Adler, Bareket Dassa, Dena Leshkowitz, Minchul Kim, Guy Bewick, Robert W Banks, Elazar Zelzerab19345, RRID:AB_444865",Section 1:300Whole mount 1:50 Antibody,Anti-MYL2(rabbit polyclonal),Abcam,"Cat# ab79935, RRID:AB_1952220",Section 1:100Whole mount 1:100 Antibody,Anti-GLUT1(rabbit monoclonal),Abcam,"Cat#

(5) Cardiac Development in the Presence of Cadmium: An in Vitro Study Using Human Embryonic Stem Cells and Cardiac OrganoidsEnviron Health PerspectNovember 2, 2022Xian Wu, Yichang Chen, Anthony Luz, Guang Hu, Erik J. Tokartranscription factor II/COUP-TFII (PP-H7147-00; Perseus Proteomics); and myosin light chain 2/MYL2 (ab79935; Abcam)] for 2 d on a thermal mixer at 300 rpm at 4°C followed by three washes in PBS and incubation

(6) A scalable and tunable thermoreversible polymer for 3D human pluripotent stem cell biomanufacturingiScienceOctober 21, 2022Johnson HJ, Chakraborty S, Muckom RJ, Balsara NP, Schaffer DV.MA5-36095; RRID:AB_2890273 Rabbit monoclonal anti-Myosin Light Chain 2v (MLC2v)(FC 1:500),Abcam,Cat#ab79935; RRID:AB_1952220 "Mouse monoclonal ant-Alpha fetoprotein (AFP)(ICC 1:200, FC 1:100)",R&D Systems,Cat#MAB1368;

(7) A Uterusâ€Inspired Niche Drives Blastocyst Development to the Early OrganogenesisAdvanced ScienceOctober 1, 2022Zhen Gu, Jia Guo, Jinglei Zhai, Guihai Feng, Xianning Wang, Zili Gao, Kai Li, Shen Ji, Leyun Wang, Yanhong Xu, et al.R&D Systems), CD31 (1:100, ab222783, Abcam), GATA4 (1:100, sc‐25310, Santa Cruz), myosin light chain (1:100, ab79935, Abcam), myosin heavy chain (1:100, 53‐6503‐82, eBioscience). The secondary antibodies were incubated

(8) Dysregulation of the Enteric Nervous System in the Mid Colon of Complement Component 3 Knockout Mice with Constipation PhenotypesInternational Journal of Molecular SciencesJune 20, 2022Yun Ju Choi, Hee Jin Song, Ji Eun Kim, Su Jin Lee, You Jeong Jin, Yu Jeong Roh, Ayun Seol, Hye Sung Kim, Dae Youn Hwang(Cell Signaling Technology Inc.; #4292), anti-p-PI3K (Cell Signaling Technology Inc.; #4228), anti-MLC (Abcam Com.; ab79935), anti-p-MLC (Abcam Com.; ab2480), anti-nNOS (Abcam Com.; ab3511), anti-NSE (Abcam Com.; ab79757), anti-C-kit

(9) Ultraefficient extracellular vesicle-guided direct reprogramming of fibroblasts into functional cardiomyocytesScience advancesFebruary 25, 2022Kim H, Song BW, Park SJ, Choi SW, Moon H, Hwang KC, Kang SW, Moon SH, Yang Y, Kwon IC, et al.mouse IgG Nkx-2.5 (ab91196), 1:200 rabbit IgG Connexin-43 (ab11370), 1:100 polyclonal rabbit IgG MLC2v (ab79935), 1:200 monoclonal mouse IgG Ki67 (ab8191, all Abcam), 1:100 monoclonal rabbit IgG α-actinin (7H1L69,

(10) Molecular and electrophysiological evaluation of human cardiomyocyte subtypes to facilitate generation of composite cardiac modelsJournal of tissue engineeringJanuary 1, 2022Li J, Wiesinger A, Fokkert L, Boukens BJ, Verkerk AO, Christoffels VM, Boink GJJ, Devalla HD.#A7811), SHOX2, 1:200 (Abcam, #ab55740), NR2F2, 1:200 (R&D Systems, #PP-H7147-00), MYL2, 1:200 (Abcam, #ab79935). Flow cytometry Cardiomyocytes were dissociated with 1x TrypLE Select (ThermoFisher #12563011) for

(11) TMEM16A inhibits angiotensin II-induced basilar artery smooth muscle cell migration in a WNK1-dependent mannerActa pharmaceutica Sinica. BDecember 1, 2021Zheng H, Li X, Zeng X, Huang C, Ma M, Lv X, Zhang Y, Sun L, Wang G, Du Y, et al.AngII-induced hypertension. 2 Materials and methods 2.1 Reagents Antibodies to MLC20 (ab76092), p-MLC20 (ab79935), MLCK (ab76092), p-MLCK (ab200809), TMEM16A (ab53213) were from Abcam Biotechnology Inc (Abcam, Cambridge,

(12) A Human 3D Cardiomyocyte Risk Model to Study the Cardiotoxic Influence of X-rays and Other Noxae in AdultsCellsSeptember 30, 2021Smit T, Schickel E, Azimzadeh O, von Toerne C, Rauh O, Ritter S, Durante M, Schroeder IS.(Badrilla, Leeds, UK, #a010-31); anti-HCN4 (Abcam, #ab66501); and anti-myosin light chain 2 (Abcam, #ab79935). The secondary antibodies Alexa594 FluorTM anti-mouse (Merck, #A32744) or anti-rabbit (Merck, #A24343)

(13) Cues from human atrial extracellular matrix enrich the atrial differentiation of human induced pluripotent stem cell-derived cardiomyocytesBiomaterials ScienceMay 18, 2021Fernanda C. P. Mesquita et al.minutes at room temperature. Primary antibodies MLC2a (BD Biosciences, cat# 565496), MLC2v (Abcam, cat# ab79935), and cTNT (Thermo Fisher Scientific Inc., cat# MA5-12960 and Abcam, cat# ab45932) were diluted in a

(14) Deficiency of macrophage PHACTR1 impairs efferocytosis and promotes atherosclerotic plaque necrosisThe Journal of clinical investigationApril 15, 2021Kasikara C, Schilperoort M, Gerlach B, Xue C, Wang X, Zheng Z, Kuriakose G, Dorweiler B, Zhang H, Fredman G, et al.ab229120, for BMDMs and HMDMs [Figure 2]), anti-GAPDH (Cell Signaling Technology, 8884S), antiMLC (Abcam, ab79935), anti-PP1 (Abcam, ab150782), and anti-MYC (Cell Signaling Technology, 2272S) diluted in PBS containing

(15) Switching between blebbing and lamellipodia depends on the degree of non-muscle myosin II activityJournal of cell scienceJanuary 13, 2021Ghosh I, Singh RK, Mishra M, Kapoor S, Jana SS.min followed by antibodies against pRLC (1:200; ThermoFisher, # MA5-15163) and RLC (1:200; Abcam, #ab79935) at 4°C overnight (Halder et al., 2019; Fu et al., 2015; Orgaz et al., 2020). Mouse and rabbit secondary

(16) Generating ring-shaped engineered heart tissues from ventricular and atrial human pluripotent stem cell-derived cardiomyocytesNature CommunicationsDecember 1, 2020Idit Goldfracht, Stephanie Protze, Assad Shiti, Noga Setter, Amit Gruber, Naim Shaheen, Yulia Nartiss, Gordon Keller, Lior Gepsteinthe unconjugated primary antibodies targeting cTnT (MA5-12960, Thermo-Fisher Scientific) and MLC-2V (ab79935, Abcam) in FACS buffer consisting of PBS with 5% fetal bovine serum (FBS, Gibco). Cells were then washed

(17) Myosin light chain kinase is a potential target for hypopharyngeal cancer treatmentBiomedicine & PharmacotherapyNovember 1, 2020Feng Cao et al.Hospital (Yenzyme Rb33), pMLC (3671 s) antibody was from cell signaling. MLC antibody was from Abcam (ab79935). Caspases3 (9662 s), cleavage caspases3 (9664 s), Bcl-xl (2764), Bax (2774s), Rock1 (4035) were from

(18) Combined Effects of Electric Stimulation and Microgrooves in Cardiac Tissue‐on‐a‐Chip for Drug ScreeningSmall MethodsOctober 9, 2020Li Ren et al.anti-α-Actinin antibody (ab210557), anti-Cardiac Troponin T (cTnT) antibody (ab8295), anti-MLC2 antibody (ab79935), Alexa Fluor 488 antibody (ab150081), Apoptosis/Necrosis Assay Kit (blue, green, red) (ab176749), and

(19) Bmi1 inhibitor PTC-209 promotes Chemically-induced Direct Cardiac Reprogramming of cardiac fibroblasts into cardiomyocytesScientific reportsApril 28, 2020Testa G, Russo M, Di Benedetto G, Barbato M, Parisi S, Pirozzi F, Tocchetti CG, Abete P, Bonaduce D, Russo T, et al.(MF20) (14-6503-82, eBioscience), DDR2 (sc-81707, Santa Cruz), CD31 (e-ab-30820, Elabscience), MLC2v (ab79935, Abcam), α-Actinin (A7811, Merck). RNA isolation and quantitative PCR RNA isolation and quantitative PCR

(20) Mylk3 null C57BL/6N mice develop cardiomyopathy, whereas Nnt null C57BL/6J mice do notLife Science AllianceApril 1, 2020Jack L Williams et al.anti-GADPH (1:2,000, Cat. no. ab9485, RRID:AB_307275; Abcam), and rabbit anti-MYL2 (1:5,000, Cat. no. ab79935, RRID:AB_1952220; Abcam). H&E Freshly isolated hearts were immersed in 4% PFA on ice overnight. Tissues

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Anti-Myosin Light Chain 2 antibody from Abcam

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Anti-Myosin Light Chain 2 antibody from Abcam

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